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gs 250 molecular imager  (Bio-Rad)


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    Structured Review

    Bio-Rad gs 250 molecular imager
    Gs 250 Molecular Imager, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gs-250+molecular+imager/Image+Lab+Touch+Software+for+ChemiDoc+Touch+Imager+Version+2%2E3/pmc04053116-63-6-10
    Average 90 stars, based on 1 article reviews
    gs 250 molecular imager - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Control of mammalian glycogen synthase by PAS kinase
    Article Snippet: The extent of phosphorylation was determined by analysis of dried gels using a Bio-Rad GS-250 Molecular Imager. fig ft0 fig mode=article f1 Fig. 5. caption a4 Phosphorylation of glycogen synthase by hPASK is specifically inhibited by glycogen. ( A ) I-form glycogen synthase from rabbit muscle was subjected to phosphorylation in the presence of [γ- 32 P]ATP by wild-type hPASK, hPASK ΔN955, or hPASK ... For histone phosphorylation, histone type III (Sigma) was used at a concentration of 0.3 mg/ml, and reactions were terminated by spotting aliquots to P-81 phosphocellulose paper (Whatman) and washing with 1% (vol/vol) phosphoric acid.

    Article Title: Tilia platyphyllos Scop.-Tuber brumale Vittad. vs. T. platyphyllos Scop.-T. borchii Vittad. ectomycorrhizal systems: a comparison of structural and functional traits.
    Article Snippet: Ectomycorrhizae are mutualistic associations of several species of fungi with higher plants.. Their formation involves alterations in the morphology and cell structure of the plant root and fungal mycelium.. These modifications are correlated with mRNA and protein synthesis in the two symbionts.

    Article Title: Diacylglycerol Kinase-? Mediates Hepatocyte Growth Factor-induced Epithelial Cell Scatter by Regulating Rac Activation and Membrane Ruffling
    Article Snippet: Radioactive signals were detected and quantified with GS-250 Molecular Imager and its Phosphor Analyst Software (Bio-Rad, Hercules, CA).

    Article Title: SAP-MEDIATED INHIBITION OF DIACYLGLYCEROL KINASE ALPHA REGULATES TCR-INDUCED DIACYLGLYCEROL SIGNALING
    Article Snippet: Radioactive signals were detected and quantified by GS-250 Molecular Imager and Phosphor Analyst Software (Bio-Rad).

    Article Title: Nitric oxide production by the vacuolar-type (H+)-ATPase inhibitors bafilomycin A1 and concanamycin A and its possible role in apoptosis in RAW 264.7 cells.
    Article Snippet: In the mouse leukemic monocyte cell line RAW 264.7, the vacuolar-type (H )-ATPase (V-ATPase) inhibitors bafilomycin A1 and concanamycin A induced nitric oxide (NO) production through the expression of inducible nitric-oxide synthase mRNA and its protein and decreased cell growth and survival as determined by 3-(4,5-dimethyl(thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.. Bafilomycin A1 and concanamycin A activated nuclear factor (NF)B and activator protein-1 and decreased the level of I Band increased that of phosphorylated c-Jun N-terminal kinase (JNK).. NO production induced by these V-ATPase inhibitors was suppressed by the NFB inhibitor Bay 11-7082 [(E)3-[(4-methylphenyl)sulfonyl])2-propenenitrile] and the JNK inhibitor SP600125 [anthra[1,9cd]pyrazol-6(2H)-one] in parallel with the partial alleviation of the V-ATPase inhibitor-induced decrease in MTT response.

    Article Title: Inhibition of translation initiation complex formation by GE81112 unravels a 16S rRNA structural switch involved in P-site decoding
    Article Snippet: The gels were dried, and the radioactivity therein was detected and quantified by a Bio-Rad model GS-250 molecular imager.

    Article Title: Negative regulation of diacylglycerol kinase theta mediates adenosine-dependent hepatocyte preconditioning.
    Article Snippet: Radioactive signals were detected and quantified by GS-250 Molecular Imager and Phosphor Analyst Software (Bio-Rad, Milan, Italy).

    Article Title: Diacylglycerol kinase-alpha phosphorylation by Src on Y335 is required for activation, membrane recruitment and Hgf-induced cell motility.
    Article Snippet: Radioactive signals were detected and quantified by GS-250 Molecular Imager and Phosphor Analyst Software (Bio-Rad).

    Acrylamide Gel Assay:

    Article Title: Control of mammalian glycogen synthase by PAS kinase
    Article Snippet: The extent of phosphorylation was determined by analysis of dried gels using a Bio-Rad GS-250 Molecular Imager. fig ft0 fig mode=article f1 Fig. 5. caption a4 Phosphorylation of glycogen synthase by hPASK is specifically inhibited by glycogen. ( A ) I-form glycogen synthase from rabbit muscle was subjected to phosphorylation in the presence of [γ- 32 P]ATP by wild-type hPASK, hPASK ΔN955, or hPASK ... For histone phosphorylation, histone type III (Sigma) was used at a concentration of 0.3 mg/ml, and reactions were terminated by spotting aliquots to P-81 phosphocellulose paper (Whatman) and washing with 1% (vol/vol) phosphoric acid.

    Article Title: Tilia platyphyllos Scop.-Tuber brumale Vittad. vs. T. platyphyllos Scop.-T. borchii Vittad. ectomycorrhizal systems: a comparison of structural and functional traits.
    Article Snippet: Ectomycorrhizae are mutualistic associations of several species of fungi with higher plants.. Their formation involves alterations in the morphology and cell structure of the plant root and fungal mycelium.. These modifications are correlated with mRNA and protein synthesis in the two symbionts.

    Article Title: Diacylglycerol Kinase-? Mediates Hepatocyte Growth Factor-induced Epithelial Cell Scatter by Regulating Rac Activation and Membrane Ruffling
    Article Snippet: Radioactive signals were detected and quantified with GS-250 Molecular Imager and its Phosphor Analyst Software (Bio-Rad, Hercules, CA).

    Article Title: SAP-MEDIATED INHIBITION OF DIACYLGLYCEROL KINASE ALPHA REGULATES TCR-INDUCED DIACYLGLYCEROL SIGNALING
    Article Snippet: Radioactive signals were detected and quantified by GS-250 Molecular Imager and Phosphor Analyst Software (Bio-Rad).

    Article Title: Nitric oxide production by the vacuolar-type (H+)-ATPase inhibitors bafilomycin A1 and concanamycin A and its possible role in apoptosis in RAW 264.7 cells.
    Article Snippet: In the mouse leukemic monocyte cell line RAW 264.7, the vacuolar-type (H )-ATPase (V-ATPase) inhibitors bafilomycin A1 and concanamycin A induced nitric oxide (NO) production through the expression of inducible nitric-oxide synthase mRNA and its protein and decreased cell growth and survival as determined by 3-(4,5-dimethyl(thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.. Bafilomycin A1 and concanamycin A activated nuclear factor (NF)B and activator protein-1 and decreased the level of I Band increased that of phosphorylated c-Jun N-terminal kinase (JNK).. NO production induced by these V-ATPase inhibitors was suppressed by the NFB inhibitor Bay 11-7082 [(E)3-[(4-methylphenyl)sulfonyl])2-propenenitrile] and the JNK inhibitor SP600125 [anthra[1,9cd]pyrazol-6(2H)-one] in parallel with the partial alleviation of the V-ATPase inhibitor-induced decrease in MTT response.

    Article Title: Inhibition of translation initiation complex formation by GE81112 unravels a 16S rRNA structural switch involved in P-site decoding
    Article Snippet: The gels were dried, and the radioactivity therein was detected and quantified by a Bio-Rad model GS-250 molecular imager.

    Article Title: Negative regulation of diacylglycerol kinase theta mediates adenosine-dependent hepatocyte preconditioning.
    Article Snippet: Radioactive signals were detected and quantified by GS-250 Molecular Imager and Phosphor Analyst Software (Bio-Rad, Milan, Italy).

    Article Title: Diacylglycerol kinase-alpha phosphorylation by Src on Y335 is required for activation, membrane recruitment and Hgf-induced cell motility.
    Article Snippet: Radioactive signals were detected and quantified by GS-250 Molecular Imager and Phosphor Analyst Software (Bio-Rad).



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